Genome sequencing and functional characterization of a Dictyopanus pusillus fungal enzymatic extract offers a promising alternative for lignocellulose pretreatment of oil palm residues.
Identifieur interne : 000078 ( Main/Exploration ); précédent : 000077; suivant : 000079Genome sequencing and functional characterization of a Dictyopanus pusillus fungal enzymatic extract offers a promising alternative for lignocellulose pretreatment of oil palm residues.
Auteurs : Andrés M. Rueda [Canada, Colombie] ; Yossef L Pez De Los Santos [Canada] ; Antony T. Vincent [Canada] ; Myriam Létourneau [Canada] ; Inés Hernández [Colombie] ; Clara I. Sánchez [Colombie] ; Daniel Molina V [Colombie] ; Sonia A. Ospina [Colombie] ; Frédéric J. Veyrier [Canada] ; Nicolas Doucet [Canada]Source :
- PloS one [ 1932-6203 ] ; 2020.
Descripteurs français
- KwdFr :
- Agaricales (classification), Agaricales (enzymologie), Agaricales (génétique), Biomasse (MeSH), Concentration en ions d'hydrogène (MeSH), Génome fongique (MeSH), Huile de palme (métabolisme), Laccase (génétique), Laccase (métabolisme), Lignine (métabolisme), Oxydoréduction (MeSH), Phylogenèse (MeSH), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Séquençage du génome entier (MeSH), Température (MeSH).
- MESH :
- enzymologie : Agaricales.
- génétique : Agaricales, Laccase, Protéines fongiques.
- métabolisme : Huile de palme, Laccase, Lignine, Protéines fongiques.
- Biomasse, Concentration en ions d'hydrogène, Génome fongique, Oxydoréduction, Phylogenèse, Séquençage du génome entier, Température.
English descriptors
- KwdEn :
- Agaricales (classification), Agaricales (enzymology), Agaricales (genetics), Biomass (MeSH), Fungal Proteins (genetics), Fungal Proteins (metabolism), Genome, Fungal (MeSH), Hydrogen-Ion Concentration (MeSH), Laccase (genetics), Laccase (metabolism), Lignin (metabolism), Oxidation-Reduction (MeSH), Palm Oil (metabolism), Phylogeny (MeSH), Temperature (MeSH), Whole Genome Sequencing (MeSH).
- MESH :
- chemical , genetics : Fungal Proteins, Laccase.
- classification : Agaricales.
- enzymology : Agaricales.
- genetics : Agaricales.
- chemical , metabolism : Fungal Proteins, Laccase, Lignin, Palm Oil.
- Biomass, Genome, Fungal, Hydrogen-Ion Concentration, Oxidation-Reduction, Phylogeny, Temperature, Whole Genome Sequencing.
Abstract
The pretreatment of biomass remains a critical requirement for bio-renewable fuel production from lignocellulose. Although current processes primarily involve chemical and physical approaches, the biological breakdown of lignin using enzymes and microorganisms is quickly becoming an interesting eco-friendly alternative to classical processes. As a result, bioprospection of wild fungi from naturally occurring lignin-rich sources remains a suitable method to uncover and isolate new species exhibiting ligninolytic activity. In this study, wild species of white rot fungi were collected from Colombian forests based on their natural wood decay ability and high capacity to secrete oxidoreductases with high affinity for phenolic polymers such as lignin. Based on high activity obtained from solid-state fermentation using a lignocellulose source from oil palm as matrix, we describe the isolation and whole-genome sequencing of Dictyopanus pusillus, a wild basidiomycete fungus exhibiting ABTS oxidation as an indication of laccase activity. Functional characterization of a crude enzymatic extract identified laccase activity as the main enzymatic contributor to fungal extracts, an observation supported by the identification of 13 putative genes encoding for homologous laccases in the genome. To the best of our knowledge, this represents the first report of an enzymatic extract exhibiting laccase activity in the Dictyopanus genera, offering means to exploit this species and its enzymes for the delignification process of lignocellulosic by-products from oil palm.
DOI: 10.1371/journal.pone.0227529
PubMed: 32730337
PubMed Central: PMC7392265
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Rueda</name><affiliation wicri:level="1"><nlm:affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval</wicri:regionArea><wicri:noRegion>Laval</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga</wicri:regionArea><wicri:noRegion>Bucaramanga</wicri:noRegion></affiliation></author><author><name sortKey="L Pez De Los Santos, Yossef" sort="L Pez De Los Santos, Yossef" uniqKey="L Pez De Los Santos Y" first="Yossef" last="L Pez De Los Santos">Yossef L Pez De Los Santos</name><affiliation wicri:level="1"><nlm:affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval</wicri:regionArea><wicri:noRegion>Laval</wicri:noRegion></affiliation></author><author><name sortKey="Vincent, Antony T" sort="Vincent, Antony T" uniqKey="Vincent A" first="Antony T" last="Vincent">Antony T. Vincent</name><affiliation wicri:level="1"><nlm:affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval</wicri:regionArea><wicri:noRegion>Laval</wicri:noRegion></affiliation></author><author><name sortKey="Letourneau, Myriam" sort="Letourneau, Myriam" uniqKey="Letourneau M" first="Myriam" last="Létourneau">Myriam Létourneau</name><affiliation wicri:level="1"><nlm:affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval</wicri:regionArea><wicri:noRegion>Laval</wicri:noRegion></affiliation></author><author><name sortKey="Hernandez, Ines" sort="Hernandez, Ines" uniqKey="Hernandez I" first="Inés" last="Hernández">Inés Hernández</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga</wicri:regionArea><wicri:noRegion>Bucaramanga</wicri:noRegion></affiliation></author><author><name sortKey="Sanchez, Clara I" sort="Sanchez, Clara I" uniqKey="Sanchez C" first="Clara I" last="Sánchez">Clara I. Sánchez</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga</wicri:regionArea><wicri:noRegion>Bucaramanga</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga</wicri:regionArea><wicri:noRegion>Bucaramanga</wicri:noRegion></affiliation></author><author><name sortKey="Molina V, Daniel" sort="Molina V, Daniel" uniqKey="Molina V D" first="Daniel" last="Molina V">Daniel Molina V</name><affiliation wicri:level="1"><nlm:affiliation>Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Escuela de Química, Universidad Industrial de Santander, Bucaramanga</wicri:regionArea><wicri:noRegion>Bucaramanga</wicri:noRegion></affiliation></author><author><name sortKey="Ospina, Sonia A" sort="Ospina, Sonia A" uniqKey="Ospina S" first="Sonia A" last="Ospina">Sonia A. Ospina</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia.</nlm:affiliation><country xml:lang="fr">Colombie</country><wicri:regionArea>Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá</wicri:regionArea><wicri:noRegion>Bogotá</wicri:noRegion></affiliation></author><author><name sortKey="Veyrier, Frederic J" sort="Veyrier, Frederic J" uniqKey="Veyrier F" first="Frédéric J" last="Veyrier">Frédéric J. Veyrier</name><affiliation wicri:level="1"><nlm:affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval</wicri:regionArea><wicri:noRegion>Laval</wicri:noRegion></affiliation></author><author><name sortKey="Doucet, Nicolas" sort="Doucet, Nicolas" uniqKey="Doucet N" first="Nicolas" last="Doucet">Nicolas Doucet</name><affiliation wicri:level="1"><nlm:affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval</wicri:regionArea><wicri:noRegion>Laval</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>PROTEO, Québec Network for Research on Protein Function, Engineering, and Applications, Québec, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>PROTEO, Québec Network for Research on Protein Function, Engineering, and Applications, Québec</wicri:regionArea><wicri:noRegion>Québec</wicri:noRegion></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (classification)</term><term>Agaricales (enzymology)</term><term>Agaricales (genetics)</term><term>Biomass (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Genome, Fungal (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Laccase (genetics)</term><term>Laccase (metabolism)</term><term>Lignin (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Palm Oil (metabolism)</term><term>Phylogeny (MeSH)</term><term>Temperature (MeSH)</term><term>Whole Genome Sequencing (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agaricales (classification)</term><term>Agaricales (enzymologie)</term><term>Agaricales (génétique)</term><term>Biomasse (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Génome fongique (MeSH)</term><term>Huile de palme (métabolisme)</term><term>Laccase (génétique)</term><term>Laccase (métabolisme)</term><term>Lignine (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Séquençage du génome entier (MeSH)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Laccase</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agaricales</term><term>Laccase</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Laccase</term><term>Lignin</term><term>Palm Oil</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Huile de palme</term><term>Laccase</term><term>Lignine</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Genome, Fungal</term><term>Hydrogen-Ion Concentration</term><term>Oxidation-Reduction</term><term>Phylogeny</term><term>Temperature</term><term>Whole Genome Sequencing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Concentration en ions d'hydrogène</term><term>Génome fongique</term><term>Oxydoréduction</term><term>Phylogenèse</term><term>Séquençage du génome entier</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pretreatment of biomass remains a critical requirement for bio-renewable fuel production from lignocellulose. Although current processes primarily involve chemical and physical approaches, the biological breakdown of lignin using enzymes and microorganisms is quickly becoming an interesting eco-friendly alternative to classical processes. As a result, bioprospection of wild fungi from naturally occurring lignin-rich sources remains a suitable method to uncover and isolate new species exhibiting ligninolytic activity. In this study, wild species of white rot fungi were collected from Colombian forests based on their natural wood decay ability and high capacity to secrete oxidoreductases with high affinity for phenolic polymers such as lignin. Based on high activity obtained from solid-state fermentation using a lignocellulose source from oil palm as matrix, we describe the isolation and whole-genome sequencing of Dictyopanus pusillus, a wild basidiomycete fungus exhibiting ABTS oxidation as an indication of laccase activity. Functional characterization of a crude enzymatic extract identified laccase activity as the main enzymatic contributor to fungal extracts, an observation supported by the identification of 13 putative genes encoding for homologous laccases in the genome. To the best of our knowledge, this represents the first report of an enzymatic extract exhibiting laccase activity in the Dictyopanus genera, offering means to exploit this species and its enzymes for the delignification process of lignocellulosic by-products from oil palm.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32730337</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>07</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>7</Issue><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Genome sequencing and functional characterization of a Dictyopanus pusillus fungal enzymatic extract offers a promising alternative for lignocellulose pretreatment of oil palm residues.</ArticleTitle><Pagination><MedlinePgn>e0227529</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0227529</ELocationID><Abstract><AbstractText>The pretreatment of biomass remains a critical requirement for bio-renewable fuel production from lignocellulose. Although current processes primarily involve chemical and physical approaches, the biological breakdown of lignin using enzymes and microorganisms is quickly becoming an interesting eco-friendly alternative to classical processes. As a result, bioprospection of wild fungi from naturally occurring lignin-rich sources remains a suitable method to uncover and isolate new species exhibiting ligninolytic activity. In this study, wild species of white rot fungi were collected from Colombian forests based on their natural wood decay ability and high capacity to secrete oxidoreductases with high affinity for phenolic polymers such as lignin. Based on high activity obtained from solid-state fermentation using a lignocellulose source from oil palm as matrix, we describe the isolation and whole-genome sequencing of Dictyopanus pusillus, a wild basidiomycete fungus exhibiting ABTS oxidation as an indication of laccase activity. Functional characterization of a crude enzymatic extract identified laccase activity as the main enzymatic contributor to fungal extracts, an observation supported by the identification of 13 putative genes encoding for homologous laccases in the genome. To the best of our knowledge, this represents the first report of an enzymatic extract exhibiting laccase activity in the Dictyopanus genera, offering means to exploit this species and its enzymes for the delignification process of lignocellulosic by-products from oil palm.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rueda</LastName><ForeName>Andrés M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López de Los Santos</LastName><ForeName>Yossef</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vincent</LastName><ForeName>Antony T</ForeName><Initials>AT</Initials><AffiliationInfo><Affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Létourneau</LastName><ForeName>Myriam</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hernández</LastName><ForeName>Inés</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sánchez</LastName><ForeName>Clara I</ForeName><Initials>CI</Initials><AffiliationInfo><Affiliation>Centro de Estudios e Investigaciones Ambientales, Universidad Industrial de Santander, Bucaramanga, Colombia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Escuela de Microbiología, Universidad Industrial de Santander, Bucaramanga, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Molina V</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Escuela de Química, Universidad Industrial de Santander, Bucaramanga, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ospina</LastName><ForeName>Sonia A</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Veyrier</LastName><ForeName>Frédéric J</ForeName><Initials>FJ</Initials><AffiliationInfo><Affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Doucet</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><Identifier Source="ORCID">0000-0002-1952-9380</Identifier><AffiliationInfo><Affiliation>Centre Armand-Frappier Santé Biotechnologie, Institut National de la Recherche Scientifique (INRS), Université du Québec, Laval, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>PROTEO, Québec Network for Research on Protein Function, Engineering, and Applications, Québec, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>5QUO05548Z</RegistryNumber><NameOfSubstance UI="D000073878">Palm Oil</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000363" MajorTopicYN="N">Agaricales</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016681" MajorTopicYN="Y">Genome, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000073878" MajorTopicYN="N">Palm Oil</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000073336" MajorTopicYN="N">Whole Genome Sequencing</DescriptorName></MeshHeading></MeshHeadingList><CoiStatement>Private funding provided by Industrias Acuña INAL LTDA (Grant number 8712) resulted from an internal competition by the Universidad Industrial de Santander (UIS) to support part of the current research project performed in the laboratory of Clara I. Sánchez at UIS (Colombia). Industrias Acuña INAL LTDA does not commercially benefit from the publication of this manuscript, nor was it involved in the conception, design, processing, analysis, or communication of the results and research project. All authors are fully committed to the full disclosure of all results and research materials resulting from the current research. All authors proclaim that this declaration of competing interests does not alter their adherence to all the PLOS ONE policies on sharing data and material.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32730337</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0227529</ArticleId><ArticleId 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